منابع مشابه
Axonemal dynein from Tetrahymena.
Axonemal dynein from Tetrahymena cilia can be separated on a sucrose gradient into two fractions, at least one of which appears to be polymorphic. We have been using immuno-electron microscopy in order to try and locate the different types of dynein molecules within the axonemal structure.
متن کاملThree-dimensional reconstruction of axonemal outer dynein arms in situ by electron tomography.
We present here for the first time a 3D reconstruction of in situ axonemal outer dynein arms. This reconstruction has been obtained by electron tomography applied to a series of tilted images collected from metal replicas of rapidly frozen, cryofractured, and metal-replicated sperm axonemes of the cecidomid dipteran Monarthropalpus flavus. This peculiar axonemal model consists of several microt...
متن کاملCorrection: Chlamydomonas DYX1C1/PF23 is essential for axonemal assembly and proper morphology of inner dynein arms
[This corrects the article DOI: 10.1371/journal.pgen.1006996.].
متن کاملTorque generation by axonemal outer-arm dynein.
Outer-arm dynein is the main engine providing the motive force in cilia. Using three-dimensional tracking microscopy, we found that contrary to previous reports Tetrahymena ciliary three-headed outer-arm dynein (αβγ) as well as proteolytically generated two-headed (βγ) and one-headed (α) subparticles showed clockwise rotation of each sliding microtubule around its longitudinal axis in microtubu...
متن کاملView Does Axonemal Dynein Push, Pull, or Oscillate?
Dynein is the molecular motor that provides motive force in cilia and flagella. Dynein is anchored to the A-subtubule of the outer doublets by a club-shaped extension called the stem, which supports the large globular head of the molecule. Dynein forms an attachment or cross-bridge to the B-subtubule of the adjacent outer doublet through a slender appendage extending from the head that is calle...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Perspectives in Biology
سال: 2016
ISSN: 1943-0264
DOI: 10.1101/cshperspect.a028100